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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 146 (1979), S. 513-520 
    ISSN: 1432-2048
    Keywords: Cotyledons (storage proteins) ; Glycoproteins ; Phaseolus ; Phytohemagglutinin ; Proteins (storage) ; Seeds (proteins) ; Vicilin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A particulate preparation from developing cotyledons of Phaseolus vulgaris L. was incubated with uridine-5′-diphospho-N-acetyl-D-glucosamine (UDP-GlcNAc; [6-3H]glucosamine), and by polyacrylamide gel electrophoretic analysis it was shown that the labeled (N-acetyl)glucosamine (GlcNAc) was incorporated into the principal reserve protein of the cotyledons, vicilin, and also into phytohemagglutinin. Some of the labeled product also reacted with antiserum to vicilin from mature seeds. In contrast it was not possible to detect the incorporation of labeled mannose from guanosine-5′-diphospho-D-mannose (GDP-mannose; [U-14C]mannose) into either of these proteins by gel-electrophoretic analysis of the mannose-labeled products, but we did observe a low incorporation of mannose into material which reacted with antiserum to vicillin. The predominant glycosylation reaction in vitro was therefore probably a transfer of GlcNAc alone, rather than in combination with mannose as preformed oligosaccharide.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 507-507 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: Your editorial in the February issue (Nat. Biotechnol. 22, 13, 2004) expressed concerns about the use of conventional crops, mainly maize, for the production of biopharmaceuticals. With the current capacity crunch for production of biopharmaceuticals by current-day technologies, and ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature biotechnology 21 (2003), S. 843-844 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: Lutz Müller-Kuhrt's commentary 'Putting nature back into drug discovery' in the June issue (Nat. Biotechnol. 21, 602, 2003) makes some very compelling arguments about the desirability of natural products research in the pharmaceutical industry. It echoes a ...
    Type of Medium: Electronic Resource
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