Crystallization and preliminary X-ray study of pig liver dihydropyrimidine dehydrogenase
International Union of Crystallography (IUCr)
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Chemistry and Pharmacology
Dihydropyrimidine dehydrogenase catalyzes the first and rate-limiting reaction in pyrimidine catabolism. The enzyme contains one FMN, one FAD and four Fe–S clusters per subunit of 1025 amino acids as prosthetic groups. It is also the major determinant of bioavailability and toxicity of 5-fluorouracil, a chemotherapeutic agent widely used in the treatment of solid tumors. Crystals of this enzyme diffracting to at least 2.5 Å have been obtained by the hanging-drop vapour-diffusion method and belong to space group P21 (unit-cell parameters a = 82.0, b = 159.3, c = 163.6 Å, β = 96.1°), with two homodimers per asymmetric unit.
Type of Medium: