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  • 1
    Electronic Resource
    Electronic Resource
    Plant cell reports 14 (1995), S. 529-533 
    ISSN: 1432-203X
    Keywords: Ancymidol ; Abscisic acid ; Paclobutrazol ; Uniconazole ; Somatic embryogenesis ; Asparagus officinalis L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of ancymidol, abscisic acid (ABA), uniconazole, and paclobutrazol on asparagus somatic embryogenesis were evaluated. Calli induced from seedlings of genotype G447 were transferred to embryo induction medium (MS plus 3% sucrose, 0.1 mg L−1 NAA, 0.5 mg L−1 kinetin and 3% gelrite), with different concentrations of these compounds. After 8 weeks, the recovered bipolar or globular embryos were placed on germination medium (MS plus 6% sucrose, 0.1 mg L−1 NAA, 0.1 mg L−1 kinetin, 0.75 mg L−1 ancymidol, 40 mg L−1 adenine sulphate dihydrate, 0.17 mg L−1 sodium phosphate monobasic and 3% gelrite) for conversion to plantlets. Inclusion of ancymidol, ABA, uniconazole and paclobutrazol in the embryo induction medium did not affect the total number of somatic embryos produced relative to the control without these compounds. However, ancymidol, ABA and uniconazole significantly improved embryo development by increasing the production of bipolar embryos 250–750% and decreasing that of globular embryos 8–35% relative to the control. The bipolar embryos produced with any of the four compounds in the embryo induction medium converted to plantlets at rates 700–1100% greater than the control. None of the globular embryos converted to plantlets. Ancymidol (0.75 mg L−1) and ABA (0.05 mg L−1) were the most effective treatments; 61 and 46 bipolar embryos g−1 callus were produced, and 38% and 37% of the bipolar embryos converted to plantlets, respectively. These results indicated that ancymidol, ABA, uniconazole and paclobutrazol significantly enhanced the production of asparagus somatic embryos and their conversion to plantlets, and ancymidol and ABA were more effective than uniconazole and paclobutrazol.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 507-507 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: Your editorial in the February issue (Nat. Biotechnol. 22, 13, 2004) expressed concerns about the use of conventional crops, mainly maize, for the production of biopharmaceuticals. With the current capacity crunch for production of biopharmaceuticals by current-day technologies, and ...
    Type of Medium: Electronic Resource
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