Cloning and expression of the mouse pseudoautosomal steroid sulphatase gene (Sts)
Nature Publishing Group
Nature Archives 1869 - 2009
[Auszug] We designed degenerate primers for the amino terminus (NFLLI-MADDLG) of rat steroid sulphatase12 and the conserved sulphatase domain (GKWHLG), and performed two rounds of amplification ‘hemi-nested’ on rat liver cDNA. After cloning and sequencing the 321-bp PCR products, various cDNA ...
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