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  • 1
    Electronic Resource
    Electronic Resource
    Chromatographia 35 (1993), S. 149-152 
    ISSN: 1612-1112
    Keywords: Thin-layer chromatography ; Mobile phase optimization ; Microcomputer ; Simplex optimization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A computer-assisted mixture design simplex method is presented for optimization of the separation of a mixture of five benzoic acid derivatives in reversephase HPTLC and twelve PTH-amino acids in normal phase HPTLC. A two-factor selectivity rectangle concept was used. The method is based on a special polynomial estimated from nine experimental runs, using the RF difference as the selection criterion, with connection to a general simplex method for optimization using a microcomputer. Excellent agreement is obtained between predicted data and experimental results, and more than half the number of experiments required in the general simplex method can be omitted.
    Type of Medium: Electronic Resource
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