Comparative biochemistry of human skin: glycosaminoglycans from different body sites in normal subjects and in patients with localized scleroderma
Blackwell Science Ltd
Blackwell Publishing Journal Backfiles 1879-2005
Objectives The aim of this investigation is to compare the relative proportions of disaccharides of chondroitinase-digestible glycosaminoglycans (GAGs) among the different body sites in control human skin and in the skin lesions of patients with localized scleroderma.Methods The disaccharide relative proportions were determined using high-performance liquid chromatography (HPLC).Results ΔDi-4S, the main disaccharide unit of dermatan sulphate (DS), was the major skin GAG disaccharide (∼70% of the total) in control skin among all different body sites studied here. In scleroderma there was an increase in the relative proportion of both ΔDi-HA, the main disaccharide unit of hyaluronic acid (HA), and ΔDi-diSB (α-ΔUA(2SO4)-1→3-GalNAc(4SO4)), derived from DS, and a decrease in ΔDi-4S, as compared with the uninvolved skin or the site-matched control skin.Conclusion DS is the major GAG species in normal skin from different body sites. In addition, our results suggest a decrease and also a structural change in DS and an increase in the proportion of HA in scleroderma skin.
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