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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Immunogenetics 19 (1984), S. 233-241 
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Male-specific antigen expression by XY teratocarcinomas PCC7 and 7′ is demonstrated first by the rejection of tumors by female but not by male mice following challenge with these cell lines. Male-specific antigen expression is confirmed by an indirect method in which females are immunized against H-Y antigen by male skin grafts. A variant of PCC7 lacking male-specific antigen expression is described. Analysis of the karyotype and of the DNA from this variant indicate that the loss of male-specific antigen expression is a result of the loss of the Y chromosome. The ability to recover variants that have lost expression of male-specific antigen opens the possibility of their selection after mutagenesis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Immunogenetics 16 (1982), S. 577-582 
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A skin grafting protocol is described for finding H-Y antigenic variants. The method is applicable regardless of the location of the structural gene(s) for this antigen (X, Y, or autosomal). Use of this protocol revealed no evidence for H-Y antigenic variation between C57BL/6J and strains 129/J, A.BY/SnJ, C3H.SW/SnJ, and LP/J.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Immunogenetics 49 (1999), S. 215-224 
    ISSN: 1432-1211
    Keywords: Key words Chicken ; Major histocompatibility complex ; Class I ; Class II ; Haplotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Although the major histocompatibility complex of chickens (encoded in the B complex) has been studied for a number of years, almost all work has focused on the White Leghorn breed. Broiler (meat-type) chickens were derived from other breeds, including Cornish and Plymouth Rock. It was our hypothesis that new B haplotypes, not previously identified in White Leghorns, might be present in lines of broiler chickens. Furthermore, alloantisera used to identify B serotypes in Leghorn lines reportedly do not work well outside the line in which they were raised, with the result that broiler B haplotypes have not been incorporated into the universal nomenclature system. Our approach was to use a panel of B alloantisera produced to identify B serotypes within a commercial broiler breeder line (designated line A). B homozygotes identified serologically were compared by B-G genotyping using restriction fragment length polymorphism analysis. Furthermore, reverse transcription-polymerase chain reaction was used to amplify variable domains of expressed B-LB and B-F genes of homozygotes of most of the B serotypes in Line A, followed by cloning and nucleotide sequence determination. Comparison of B-LB and B-F sequences with standard Leghorn haplotypes demonstrated the existence of new alleles of B-L and B-F in a broiler breeder line, as well as the presence of alleles previously identified in Leghorns. In some cases, Leghorn-type alleles were in linkage with different B-G alleles in the broiler line than the common haplotypic associations found in Leghorn lines.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 52 (1983), S. 226-234 
    ISSN: 1432-1106
    Keywords: Transganglionic degeneration ; Dental afferents ; Trigeminal system ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Transganglionic degeneration in the trigeminal main sensory nucleus (MSN) and pars interpolaris (PI) was studied in cats following dental lesions. At early survival times, three types of terminal alteration were seen in both MSN and PI: (1) flocculent degeneration, (2) neurofilamentous hyperplasia and, (3) glycogen accumulation. With longer survival times, the magnitude of these terminal alterations increases. Electron dense degeneration was only seen in the ventral half of PI. Phagocytosis of the altered terminals was also observed. The study suggests a plausible explanation for the variations observed in the CNS projection of primary afferents with degeneraton and with HRP transport studies.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 49-55 
    ISSN: 1432-2242
    Keywords: Chloroplast DNA ; Medicago sativa ; Maternal plastid transmission ; Biparental plastid inheritance ; Heteroplasmy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A high frequency of paternal plastid transmission occurred in progeny from crosses among normal green alfalfa plants. Plastid transmission was analyzed by hybridization of radiolabeled alfalfa plastid DNA (cpDNA) probes to Southern blots of restriction digests of the progeny DNA. Each probe revealed a specific polymorphism differentiating the parental plastid genomes. Of 212 progeny, 34 were heteroplastidic, with their cpDNAs ranging from predominantly paternal to predominantly maternal. Regrowth of shoots from heteroplasmic plants following removal of top growth revealed the persistence of mixed plastids in a given plant. However, different shoots within a green heteroplasmic plant exhibited paternal, maternal, or mixed cpDNAs. Evidence of maternal nuclear genomic influence on the frequency of paternal plastid transmission was observed in some reciprocal crosses. A few tetraploid F1 progeny were obtained from tetraploid (2n=4x=32) Medicago sativa ssp. sativa x diploid (2n=2x=16) M. sativa ssp. falcata crosses, and resulted from unreduced gametes. Here more than the maternal genome alone apparently functioned in controlling plastid transmission. Considering all crosses, only 5 of 212 progeny cpDNAs lacked evidence of a definitive paternal plastid fragment.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Active spreading ridges in the North Fiji Basin range from well-developed stable ridges where largescale mantle upwelling is in progress to proto-ridges where spreading is incipient. South of 17°S, where the central ridge of the North Fiji Basin has a bathymetric profile normally expected of a fast-spreading, steadystate mid-ocean ridge, basalts are evolved N-type MORBs. North of 17°S, where the central ridge is propagating northward into old North Fiji Basin crust and spreading is in the initial stages, two types of basalt have been recovered: N-type MORBs from this northern arm of the central ridge are believed to be samples of older North Fiji Basin crust; basalts with transitional alkalic chemistry (up to 0.5% Ne in the Norm) and characterized by strong relative enrichments in Rb, Ba, K, Nb, La, Ce, Sr, P, Zr, and Ti are believed to be associated with incipient rifting. Among the latter group are compositions that are intermediate between transitional alkalic types and MORBs and these are geochemically similar to the back-arc basin (BABB) magma type defined by Sinton and Fryer (1987) from a study of Mariana back arc basin basalts. Dredges along the South Pandora Ridge, a transform zone characterized by short spreading segments, are dominated by basalts that are enriched in large-ion lithophile and high field strength minor and trace elements and compositions range from types resembling ocean island tholeiites to transitional alkalic varieties. Basalts from Rotuma are regarded as alkalic end-members of the South Pandora Ridge magmatic spectrum. In areas of the North Fiji Basin where relatively fast spreading must be accompanied by largescale asthenospheric upwelling, depleted (N-type) MORBs dominate, whereas in areas of slow mantle upwelling, or where some other tectonic effect (e.g. a transform fault) causes a transient thermal disturbance within the lithosphere or upper asthenosphere, enriched (alkalic) magmas either dominate or make a significant and noticeable contribution to the overall chemical characteristics of basalts being erupted. The MORBs have a depleted asthenospheric source, and the alkalic component is believed to derive from an enriched lithospheric or shallow asthenospheric source. The BABB magma type may simply be part of the spectrum of mixed magmas that can occur in the transitional tectonic settings represented by the early development of most back-arc basins.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 34 (1978), S. 1378-1379 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous studies show that the suppression of gonadal function in blind-anosmic rats is dependent on the pineal gland. The present results demonstrate that in young female rats both the pineal gland and dual sensory deprivation have additional independent antigonaldal effects.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 39 (1983), S. 1236-1246 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 156 (1999), S. 557-589 
    ISSN: 1420-9136
    Keywords: Key Words: Elastic wave scattering, nonlinear inversion, Rayleigh, Mie approximation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —We investigate a new nonlinear inversion method for low frequencies to determine the bulk and shear modulus as well as the material density and the location of subsurface inhomogeneities. The solution is a direct exact nonlinear inversion of single scattered waves containing near- and far-field terms for incident P and scattered P and S waves, allowing for inversion of parameters in the vicinity and at distance from the sources and receivers. Because the approach is based on single scattering theory, the range of application includes single strong scattering anomalies of various sizes like magma chambers, gas- or fluid-filled cavities, or buried near-surface obstacles. The replacement of the material properties by a new set of parameters, referred to as scattering factors, allows the inverse problem to be solved analytically. The nonlinear nature of the scattering problem is investigated and implications for the inversion process are discussed. The deviations in the elastic parameters as a function of the scattering factors show a strong asymmetry about zero, and therefore linearized approximations will perform differently, depending on the sign of the perturbation. Based on the low frequency (Rayleigh) approximation, we introduce and evaluate a pair of approximations (Mie) derived by numerical and analytical integration of the Rayleigh approximation. Both approximations are based on the underlying principle of subdividing the inhomogeneities into a number of small noninteracting parts and subsequent integration over the total volume, thus increasing the Rayleigh limit and producing better resolution of the parameter estimates during the inversion. The two Mie approximations, when evaluated as a function of scattering angle and distance, produce similar results in the mid- and far-field of the inhomogeneity and reveal better resolution than the Rayleigh approximation. For three anomalies of ± 50% in bulk modulus, shear modulus, and density, the relative error between the exact solution and the two Mie approximations remains below 10%, 20%, and 30%, respectively, for values of k p R 〈 3.0, where R is the radius of the heterogeneity. However, smaller errors for individual cases are found for values up to k p R≈ 4.5. The performance of the inversion based on the analytically and the numerically integrated Mie approximation is tested for single parameter perturbations, revealing reliable and stable inversion results for the bulk and the shear modulus, reasonable results for the density, and crosstalk between the shear modulus and the density. The results show well-defined locations of the anomalies and slight deviations in the estimates of their magnitudes, which can be explained by amplitude and phase deviations between the analytical solution used for forward modeling and the approximations used for the inversion. The analytical Mie approximation provides a fast means to estimate elastic parameters compared to the more time consuming numerically integrated approximation, while the latter can be applied to more arbitrarily shaped inhomogeneities.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 16 (1960), S. 532-534 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Die Strukturaufklärung von Cylindrocarpin (II) und Cylindrocarpidin (IV) zeigt, dass diese zwei Alkaloide die ersten Mitglieder der Aspidosperminfamilie (I) sind, in denen eine sauerstoffhaltige Seitenkette anstatt der gewöhnlichen C-5-Äthylgruppe vorliegt.
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