Statistical scanning method for the optimization of gradient elution high performance liquid chromatography
Column liquid chromatography
Optimal mobile phase
Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Summary A computer-assisted method is presented for the optimization of separation in gradient elution reversed-phase HPLC. The method is based on a polynomial estimation from nine preliminary experiments according to a two-factor (initial solvent composition C and gradient time T) rectangular design. This is followed by a two-dimension computer scanning technique. Resolution is used as the selection criterion. Good agreement was obtained between predicted data and experimental results.
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