Densitometric determination of unsaturated fatty acids with p-hydroxybenzaldehyde by HPTLC
High performance thin-layer chromatography
Unsaturated fatty acids
Analytical Chemistry and Spectroscopy
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
A rapid method for the detection and quantltatlve determlnation of unsaturated fatty acids by TLC is presented. After separation on HPTLC-RP18-plates, reaction wlth p-hydroxybenzaldehyde is carried out by a dipping procedure. Nanogram amounts of unsaturated fatty acids can be determined with good reproducibility.
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