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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 38 (1994), S. 187-190 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Optimal mobile phase ; Gradient elution ; Microcomputer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A computer-assisted method is presented for the optimization of separation in gradient elution reversed-phase HPLC. The method is based on a polynomial estimation from nine preliminary experiments according to a two-factor (initial solvent composition C and gradient time T) rectangular design. This is followed by a two-dimension computer scanning technique. Resolution is used as the selection criterion. Good agreement was obtained between predicted data and experimental results.
    Type of Medium: Electronic Resource
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